CRISPR/Cas9 genome editing in wheat

RNA-Guided Genome Editing for Target Gene Mutations in Wheat

The clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system has been used as an efficient tool for genome editing. We report the application of CRISPR-Cas-mediated genome editing to wheat (Triticum aestivum), the most important food crop plant with a very large and complex genome. The mutations were targeted in the inositol oxygenase (inox...

Optimizing multiplex CRISPR/Cas9-based genome editing for wheat

Background: CRISPR/Cas9-based genome editing holds a great promise to accelerate the development of improved crop varieties by providing a powerful tool to modify genomic regions controlling major agronomic traits. The effective deployment of this technology for crop improvement still requires further optimization of the CRISPR/Cas9 system for different applications. Here we have optimized mult...

Title: Optimizing multiplex CRISPR/Cas9-based genome editing for wheat

not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/051342 doi: bioRxiv preprint first posted online May. 2, 2016;

Genome Editing



Advancing Chimeric Antigen Receptor-Engineered T-Cell Immunotherapy Using Genome Editing Technologies: Challenges and Future Prospects

Chimeric antigen receptor engineered-T (CAR-T) cells also named as living drugs, have been recently known as a breakthrough technology and were applied as an adoptive immunotherapy against different types of cancer. They also attracted widespread interest because of the success of B-cell malignancy therapy achieved by anti-CD19 CAR-T cells. Current genetic toolbox enabled the synthesis of CARs ...